![](https://parts.igem.org/images/partbypart/icon_plasmid_backbone.png)
Plasmid_Backbone
Part:BBa_K1480009:Design
Designed by: Roberto Alejandro Valderrama Alfaro Group: iGEM14_UANL_Mty-Mexico (2014-10-17)
pSB1C3 + MID and BF TALEN (K1480001) Target sites
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2144
Illegal XbaI site found at 2159
Illegal SpeI site found at 1
Illegal PstI site found at 15 - 12INCOMPATIBLE WITH RFC[12]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2144
Illegal SpeI site found at 1
Illegal PstI site found at 15
Illegal NotI site found at 8
Illegal NotI site found at 2150 - 21INCOMPATIBLE WITH RFC[21]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2144
Illegal XhoI site found at 1080
Illegal XhoI site found at 2020 - 23INCOMPATIBLE WITH RFC[23]Illegal prefix found at 2144
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found at 2144
Plasmid lacks a suffix.
Illegal XbaI site found at 2159
Illegal SpeI site found at 1
Illegal PstI site found at 15
Illegal AgeI site found at 2945
Illegal AgeI site found at 3057 - 1000INCOMPATIBLE WITH RFC[1000]Plasmid lacks a prefix.
Plasmid lacks a suffix.
Design Notes
Modifications where inserted by PCR on both directions due to TALEN specificity.
Source
Derived from pSB1C3 plasmid